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Fluorescence images were obtained with confocal microscope Nikon A1Rsi (Nikon, Germany) microscope with Plan Plan Apo 10x/0.45 DIC N1, Plan Apo VC 20x/0.75 DIC N2, Apo 40x/1.25 WI λS DIC N2, Plan Apo VC 60x/1.4 Oil DIC N2 and Plan Apo VC 100x/1.4 Oil DIC N2 lenses and with Nikon NIS Elements AR 5.21.01 64-bit software (Nikon, Germany) or with confocal microscope LEICA Stellaris 8 (Leica Camera, Germany) with HC PL APO 20x/0.75 CS2 Air, HC PL IRAPO 40x/1.10 W CORR, PL APO 63x/1.20 W CORR CS2 or PL APO 63x/1.20 W CORR CS2 lenses and with Leica LAS X Office 1.4.7 64-bit software (Leica Camera, Germany) or with light sheet fluorescence microscopy (LSFM) ZEISS Lightsheet 7 (Zeiss, Germany) with W Plan-APO 20x/1,0 DIC lens, in water chamber and with ZEN Black 3.1 software for imaging and ZEN Blue 3.1 software for data analysis (Zeiss, Germany)

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In another approach, Narouiepour et al