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More importantly, Akt3(C119S)—sensing defective but otherwise functional kinase—was hypomorphic for all downstream phenotypes, ruling out the possibilities of off-target effects.(512) An important advantage of T-REX over other profiling methods is that the discovery efforts can be streamlined with a unique means to validate the identified sensor robustly.(512,959) Despite the powerful capabilities of chemoproteomic platforms to identify redox sensors and pin-point sites of modification on these targets, these methods do not allow modification selectively of the identified targets for functional validation