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After blocking with 5% bovine serum albumin (BSA), PVDF membranes were incubated with primary antibodies, including anti-DUSP1 (Abcam, #ab195261), anti-COXI (bs-3953R), anti-p-JNK (Abcam, # ab76572), anti-JNK (Abcam, #ab307802), anti-BAX (Cell Signaling Technology, #2772), anti-cGAS (Cell Signaling Technology, #31659), anti-STING (Cell Signaling Technology, #13647), anti-Cytochrome C (Santa Cruz, sc-13156), and anti-COX IV(Abcam, #ab16056) overnight at 4 °C

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Their motions sometimes appear noticeable even within minutes, particularly when a satellite transits the planet’s face or lies near the limb