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The second group was given vitamin E at a dose of 250 mg/kg BW/day, the third group was given vitamin C at a dose of 250 mg/kg BW/day, the fourth group was given copper nanoparticles at a dose of 100 mg/kg BW/day, the fifth group was given copper nanoparticles at a dose of 100 mg/kg BW/day and vitamin E at a dose of 250 mg/kg BW/day, the sixth group was given copper nanoparticles at a dose of 100 mg/kg BW/day and vitamin C at a dose of 250 mg/kg BW/day, and the seventh group was given copper nanoparticles at a dose of 100 mg/kg BW/day and both vitamin E and vitamin C at a dose of 250 mg/kg BW/day